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Invasion abilities of HTR-8/SVneo cells exposed to siPTTG1.

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posted on 2016-02-22, 09:24 authored by Seung Mook Lim, Hee Yeon Jang, Ji Eun Lee, Joong Sik Shin, Sun-Hwa Park, Bo Hyun Yoon, Gi Jin Kim

(A) Images and numbers of invaded HTR-8/SVneo cells after siPTTG1 treatment (original magnification, ×100; scale bar = 80 μm). (B) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells analyzed by zymography. (C) Expression of MMP-9 and MMP-2 mRNA in HTR-8/SVneo cells analyzed by qRT-PCR. (D) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells after siPTTG1 treatment analyzed by western blot. Values are means ± standard errors. *p < 0.05 and **p < 0.001. β-actin or GAPDH was used as a loading control.

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