Intracellular killing assay of antibiotics

A549 cells (1 × 10cells/well) were infected for 1 h at 37°C with 1 × 10CFU of YS001 (A) or YS005 (B) in the absence of antibiotics. After a 1-h infection followed by a 1-h incubation period with GEN (100 μg/ml), the cell culture was incubated in the presence or absence (control) of AMP, AZM, CLR, CFPN, or LVX (1 μg/ml). Two hours later, the surviving intracellular bacteria were enumerated by plating. Data represent the means ± SD of three experiments with triplicate assays. *, < 0.05 (compared with control). **, < 0.01 (compared with control). ND, not detected.<p><b>Copyright information:</b></p><p>Taken from "Comparative efficacies of different antibiotic treatments to eradicate nontypeable infection"</p><p></p><p>BMC Infectious Diseases 2008;8():15-15.</p><p>Published online 7 Feb 2008</p><p>PMCID:PMC2276502.</p><p></p>