ao8b00916_si_002.avi (3.57 MB)
Ink-Jet Printing-Assisted Modification on Polyethersulfone Membranes Using a UV-Reactive Antimicrobial Peptide for Fouling-Resistant Surfaces
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posted on 2018-08-08, 08:13 authored by Gunasekaran Mohanraj, Canwei Mao, Asatryan Armine, Roni Kasher, Christopher J. ArnuschAntimicrobial
peptides (AMPs) are promising candidates for surface
coatings to control biofilm growth on water treatment membranes because
of their broad activity and the low tendency of bacteria to develop
resistance to AMPs. However, general and convenient surface modification
methods are limited, and a deeper understanding of the antimicrobial
mechanism of action is needed for surface-attached AMPs. Here, we
show a method for covalently attaching AMPs on porous ultrafiltration
membranes using ink-jet printing and provide insight into the mode
of action for the covalently tethered peptide RWRWRWA-(Bpa) (Bpa,
4-benzophenylalanine) against Pseudomonas aeruginosa. AMP-coated ultrafiltration membranes showed surface antibacterial
activity and reduced biofilm growth. Fluorescence microscopy analysis
revealed that the modified surfaces could cause cell membrane disruption,
which was seen by live uptake of propidium iodide stain, and scanning
electron microscopy images showed compromised cell membranes of attached
bacteria. This study indicated that the mode of action of covalently
tethered AMPs was similar to that of freely soluble AMPs. The deeper
understanding of the mode of action of AMPs covalently attached to
surfaces could lead to a more rational approach for designing surfaces
with antibacterial activity.
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propidium iodide stainPolyethersulfone Membranes4- benzophenylalanineUV-Reactive Antimicrobial PeptideFouling-Resistant Surfaces Antimicrobial peptidesAMP-coated ultrafiltration membranesFluorescence microscopy analysiscontrol biofilm growthultrafiltration membranescell membranesAMPs covalentlysurface-attached AMPsRWRWRWAPseudomonas aeruginosaantimicrobial mechanismbiofilm growthwater treatment membranesscanning electron microscopy imagescell membrane disruptionsurface modification methodssurface coatingsmodeInk-Jet Printing-Assisted Modification
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