Inhibitory effects of lupene-derived pentacyclic triterpenoids from <i>Bursera simaruba</i> on HSV-1 and HSV-2 <i>in vitro</i> replication

<div><p>The cytotoxicity and antiviral properties of <i>Bursera simaruba</i> against herpes simplex viruses (HSV-1 and HSV-2) were investigated through a bioactivity-guided isolation protocol. The plant material was fractionated using solvent-solvent partitioning, size-exclusion and thin-layer chromatography. The antiviral compounds present in the most active fractions were identified by means of LC-MS and NMR. Three different methods were compared during the evaluation of antiviral activity of samples. Four lupene-related pentacyclic triterpenes were found to be responsible for the anti-herpesvirus effects of <i>B. simaruba</i> and were isolated from this species for the first time. The selective indexes (SI) of <i>B. simaruba</i>-derived samples ranged from 7.7 to 201.9.</p></div>