Inhibition of Wnt1 suppresses migration and invasion of A549 and H1299 cells.

(A) Three siRNA were designed and Wnt1 protein level was detected by western blot to select the effective siRNA for the target protein. siRNA#2 exhibited greatest inhibition of Wnt1 protein and was further validated in A549 cells. β-actin was used as a loading control. (B) Both A549 and H1299 cells transfected with siRNA-Wnt1 resulted in inhibition of cell migration using wound-healing assay. (C) Transfection of A549 and H1299 cells with siRNA-Wnt1 inhibited cell invasion using transwell migration assays. Images were captured at 200× optical magnification.