Infection of cells with virus particles that lack protein VII.

A549 cells (A) or HeLa cells (B) on glass coverslips were infected with 200 particles/cell wild-type Ad5 or the Ad5-VII-loxP virus grown in 293 cells (Ad5-VII⁺), or 2000 particles/cell VII⁻virus (Ad5-VII^–). At 24 hours post-infection, cells were processed for IF using antibodies directed against protein VII (FITC secondary antibody; left column) and Ad DNA binding protein (TRITC secondary antibody; center column). Merged images are shown in the right column. The white bar represents 10μM.