Infection of cells with virus particles that lack protein VII.

<p>A549 cells (A) or HeLa cells (B) on glass coverslips were infected with 200 particles/cell wild-type Ad5 or the Ad5-VII-loxP virus grown in 293 cells (Ad5-VII<sup>+</sup>), or 2000 particles/cell VII<sup>−</sup>virus (Ad5-VII<sup>–</sup>). At 24 hours post-infection, cells were processed for IF using antibodies directed against protein VII (FITC secondary antibody; left column) and Ad DNA binding protein (TRITC secondary antibody; center column). Merged images are shown in the right column; nuclei were visualized using DAPI staining. The white bar represents 10μM.</p>