Induction of E-cadherin expression and inhibition of HDACs by hit compounds in PANC-1 cells.

A. Western blot analysis of E-cadherin in PANC-1 cells that were exposed to two different concentrations of cluster#1 and 2 compounds. Concentrations for compound 150 were 0.5 and 1 μM. B. Western blot analysis of E-cadherin in PANC-1 cells that were exposed to 25 μM of cluster#2 compounds for 24 hrs. C. Western blot analysis in PANC-1 and BxPC-3 cells for E-cadherin, N-Cadherin, and Snail. Cells were treated with BSI (25 μM) for 24hrs. D. Western blot analysis in PANC-1 and BxPC-3 cells for H2A-Lys5, H3-Lys9, and H4-Lys8. Cells that were exposed to BSI (25 μM) for 24hrs.