Increased ROS levels and inflammatory responses in anxa2-/- mice with polymicrobial sepsis.

(A) ROS levels were detected in PMs from anxa2-/- mice pre-injected with rotenone, DPI, APO, and NAC, respectively. (B) PMs from WT and anxa2-/- mice were subjected to qPCR to detect the NOX1, NOX3, and NOX4 expression. (C) Mice were pretreated with H2O2 or DPI and at 12 h or 24 h post-CLP, ROS levels in PMs were detected. (D) IL-17A in peritoneal lavage was assayed by ELISA. (E) At 24 h post-CLP, colon tissues were stained for histological analysis. Ly6G and IL-17A were used to detect immune cells recruitment and inflammatory cytokine secretion. Data are representative of three experiments and expressed as means+SD from triplicate. One-way ANOVA (Tukey’s post hoc); *, p<0.05; **, p<0.01. Scale bar = 5 μm.