ac6b04953_si_003.mpg (13.73 MB)
Improving Sample Distribution Homogeneity in Three-Dimensional Microfluidic Paper-Based Analytical Devices by Rational Device Design
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posted on 2017-04-12, 00:00 authored by Giorgio
Gianini Morbioli, Thiago Mazzu-Nascimento, Luis Aparecido Milan, Amanda M. Stockton, Emanuel CarrilhoPaper-based
devices are a portable, user-friendly, and affordable
technology that is one of the best analytical tools for inexpensive
diagnostic devices. Three-dimensional microfluidic paper-based analytical
devices (3D-μPADs) are an evolution of single layer devices
and they permit effective sample dispersion, individual layer treatment,
and multiplex analytical assays. Here, we present the rational design
of a wax-printed 3D-μPAD that enables more homogeneous permeation
of fluids along the cellulose matrix than other existing designs in
the literature. Moreover, we show the importance of the rational design
of channels on these devices using glucose oxidase, peroxidase, and
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
reactions. We present an alternative method for layer stacking using
a magnetic apparatus, which facilitates fluidic dispersion and improves
the reproducibility of tests performed on 3D-μPADs. We also
provide the optimized designs for printing, facilitating further studies
using 3D-μPADs.
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wax-printed 3 D -μPADABTSoptimized designslayer devicesglucose oxidaseThree-Dimensional Microfluidic Paper-Based Analytical Devicesalternative methodRational Device Design Paper-based devicescellulose matrixSample Distribution Homogeneity3 D -μPADssample dispersionThree-dimensional microfluidic paper-basedfluidic dispersionlayer treatment
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