Impact of anti-vector immunity on transgene expression.
(A) Anti-vector reactivity and (B) expression of ITS01 is shown for the six animals of Fig 1. Animals with low (A10V030; orange) and intermediate (A10V026; purple) pre-existing anti-vector immunity are highlighted. After 6 months, two of these animals were re-injected with 1 x 1013 gc AAV8-ITS01 in the right quadriceps. (C) Anti-AAV8 reactivity was boosted, however (D) no increase in serum ITS01 was observed. Anti-AAV8 measurements are in arbitrary optical density (OD450nm)/ml units.