Immunocytochemical analysis of the localization of neuronal marker proteins.

Representative images and fluorescence intensity of the cellular localization of NF-H (A and B), Ascl1 (C and D), and NeuroD2 (E and F) of DFATs (n = 10 cells, random selected ×20 fields from triplicate samples), with (upper panels) or without (control; lower panels) ATRA treatment after 0, 14, and 28 days. Data are shown as the mean ± standard error of three independent experiments. *P < 0.05, compared with 0 day.