Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes
presentationposted on 03.07.2019, 12:42 by Chas Nelson, Charlotte Buckley, John J Mullins, Martin A Denvir, Jonathan M Taylor
How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.
Presented at SPIE BiOS, 2018, San Francisco, California, United States. Paper 104991F.
Yellow frames indicate animations/videos.
Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heart
British Heart FoundationFind out more...