20180131_spie-photonics-west-bios.pdf (13.28 MB)
Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes
Version 2 2019-07-03, 16:37
Version 1 2019-07-03, 12:42
presentation
posted on 2019-07-03, 12:42 authored by Chas NelsonChas Nelson, Charlotte Buckley, John J Mullins, Martin A Denvir, Jonathan M TaylorHow do you use imaging to analyse the development of the heart, which
not only changes shape but also undergoes constant, high-speed,
quasi-periodic changes? We have integrated ideas from prospective and
retrospective optical gating to capture long-term, phase-locked
developmental time-lapse videos. In this paper we demonstrate the
success of this approach over a key developmental time period: heart
looping, where large changes in heart shape prevent previous prospective
gating approaches from capturing phase- locked videos. We use the
comparison with other approaches to in vivo heart imaging to highlight
the importance of collecting the most appropriate data for the
biological question.
Presented at SPIE BiOS, 2018, San Francisco, California, United States. Paper 104991F.
Yellow frames indicate animations/videos.
Funding
Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heart
British Heart Foundation
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