Images from Chandler, Chari et al. 2017 PLoS Genetics "How well do you know your mutation? Complex effects of genetic background on expressivity, complementation, and ordering of allelic effects"

2018-03-02T18:51:05Z (GMT) by Ian Dworkin Gayatri Sivaratnam
These are the images associated with the data collected for

http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007075

For the zipped folder GS_data:
Data Provenance (how data was collected):
via dissections, area imaging
Author:
Performed - Ian Dworkin, Gayatri Sivaratnam, Samiksha Kaul
Dissections - Gayatri Sivaratnam, Samiksha Kaul
Imaging - I. Dworkin, G. Sivaratnam, Samiksha Kaul

Dates
samples Collected: July 21, 2015
Dissection: August 17 - September25, 2015
Imging: September 30, 2015 (ID, GS, SK)
(Date samples were collected, dates for imaging)

A bit about the experiment:
16 DGRP lines were crossed to 3 mutations (sd 1, sd E3, sd 58d) and a wildtype (SAM)
The effects of intermediate mutation on different lines, whether the severity of this effect varied across the different lines is being investigated.

Microscope, camera used. Leica m125 with a Leica DFC400 camera, using Leica Application Software 3.4.0. Greyscale images.

Each image is 1392 x 1040 px. Real size 2.59 x 1.93 mm

Magnification : 50X (0.5X?)
aperture about halfway across (59%)
Brightness: 68%
Gamma: 0.80

Notes:
naming convention for images: SAM/sd1/sde3/sd58d_line#_M_1/2/3/4/5/6.TIF

SAM/sd1/sde3/sd58d - either the wild type (SAM) allele for scalloped, or allele 1, E3 or 58d