EutQ-QII-Opt1-B5-280211.jpg (76.83 kB)
Image of a CD1925 protein crystal.
Image of Clostridium difficile CD1925, EutQ, protein crystal.
Methods
CD1925(17–157)-His was concentrated to 8 mg/ml in buffer A and crystallised by hanging drop vapour diffusion in drops of 1 µl protein plus 1 µl crystallisation solution over 1 ml of the latter. Crystals were obtained in 100 mM sodium acetate pH 4.5, 35% (w/v) PEG 6000, 200 mM MgCl2, these were harvested by transfer to a cryoprotection solution containing the well solution supplemented with 20% v/v PEG300 and flash cooled in liquid nitrogen. All crystallographic data were collected on beamlines I02 and I04 at Diamond Light Source (Didcot, UK) at 100 K using ADSC CCD detectors.
