figshare
Browse
gnpl_a_904306_sm5040.docx (40.94 kB)

Identification of α-glucosidase inhibitors from the leaves of Pluchea indica (L.) Less., a traditional Indonesian herb: promotion of natural product use

Download (0 kB)
Version 3 2014-08-18, 13:17
Version 2 2014-08-18, 13:17
Version 1 2014-08-18, 13:17
journal contribution
posted on 2014-08-18, 13:17 authored by Ines Septi Arsiningtyas, Maria D.P.T. Gunawan-Puteri, Eisuke Kato, Jun Kawabata

A promising approach for treating diabetes mellitus (DM) is to decrease postprandial hyperglycaemia by suppressing carbohydrate digestion using α-glucosidase inhibitors. Pluchea indica leaf extracts possess inhibitory activity against intestinal maltase. Enzyme assay-guided fractionation by chromatography yielded five active caffeoylquinic acid derivatives (15). Their structures were elucidated by mass spectrometry and NMR analysis and completed by comparison with reference data. 3,5-Di-O-caffeoylquinic acid (1), 4,5-di-O-caffeoylquinic acid methyl ester (2), 3,4,5-tri-O-caffeoylquinic acid methyl ester (3), 3,4,5-tri-O-caffeoylquinic acid (4) and 1,3,4,5-tetra-O-caffeoylquinic acid (5) were isolated. Comparison of the activities of each isolate suggested that both methyl esterification of quinic acid and the number of caffeate groups in the molecule were important for the inhibitory activity. This study provides basic information for further examination of the suitability of P. indica as a functional food and medicinal supplement for the treatment and prevention of diabetes.

History

Usage metrics

    Natural Product Research

    Categories

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC