Identification of SOD forms in wheat leaves.

Native polyacrylamide gel electrophoresis (PAGE) of SODs in 6-day-old wheat leaves: Lane 1A –etiolated, Lane 1B –etiolated and then deetiolated under white light (100 μmol photons m^-2 s^-1) for 4 hours, Lane 1C –grown under a 14 hour photoperiod (100 μmol photons m^-2 s^-1). The inhibitors were: NaCN–the inhibitor of Cu/Zn-SOD (Lanes 2A-2C) and H₂O₂ –the inhibitor of Fe-SOD and of Cu/Zn-SOD (Lanes 3A-3C). Each well was loaded with 25 μg of protein.