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Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in -4
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posted on 2011-12-31, 09:24 authored by Stefanie Link, Karin Schmitt, Dagmar Beier, Roy GrossCopyright information:
Taken from "Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in "
http://www.biomedcentral.com/1471-2180/7/100
BMC Microbiology 2007;7():100-100.
Published online 7 Nov 2007
PMCID:PMC2225982.
DNase I footprint experiments were performed on a 312 bp BamHI-HindIII DNA fragment from plasmid pSK-FP labelled at its BamHI site containing the entire upstream sequence including all four putative binding sites (BS1–BS4). The 5'-labelled probe was incubated with 0.5, 1.0, 2.0, 3.0, 4.0, 6.0 and 8.0 μg of phosphorylated BvgA(lanes 4–10, respectively). No protein was added to the reaction mixture loaded in lane 3. Lane 1 and 2 are G+A sequencing reactions on the DNA probe used as a size marker [42]. Numbers on the left indicate the distance from the translational start codon of the gene. The positions of the start sites of transcripts P1–P3 are indicated.