IR56d anterior projections are dispensable for PER to FAs.

(A) IR56d and Gr64f neurons visualized with IR56d-GAL4 driving RFP and Gr64f-LexA driving GFP. Co-localization is detected in the posterior projections, but is minimal in anterior projections. (B) Driving the GAL4 repressor Gal80 with Gr64f-LexA limits GFP expression to the non-overlapping IR56d anterior projections. (C) Restricting TNT expression to the non-overlapping anterior projection neurons does not significantly impact PER to sugar or HxA (impTNT n = 23; TNT n = 22). Error bars indicate SEM. Wilcoxon Rank Sum Test Sucrose: p>0.98; HxA: p>0.96. (D) Restricting TRPA1 expression to the non-overlapping neurons abolishes PER compared to control in which TRPA1 is driven in all IR56d neurons. Fisher’s Exact Test with Bonferroni correction for multiple comparisons; ***p<0.001.