IL-6 and RIG-I contribute additively to the DNA-induced STING degradation.

(A) qRT-PCR assessment of expression of indicated interleukins after MRC-5 cells were transfected with pcDNA3.1 (0.05 μg) for 9 hours. (B) ELISA analysis of IL-6 protein levels in the supernatants of cells at different time points. (C) qRT-PCR analysis of IL-6 mRNA at different time points. (D) Western blotting assessment of STING expression after MRC-5 cells were treated with IL-6R antibody with pcDNA3.1 transfection. (E) Western blotting assessment of STING expression after application of IL-6R antibody and siRIG-I. (F) qRT-PCR (up) and ELISA analysis (down) of IL-6 expression after STING knockdown. Asterisk (*), P <0.05.