Fig 8.tif (545.04 kB)
Hydrolysis of rhodamine-conjugated Ub-like substrates by chUSP1FL /chUAF1.
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posted on 2017-11-01, 17:32 authored by Hainan Zheng, Mengyun Wang, Chengcheng Zhao, Shanli Wu, Peifeng Yu, Yan Lü, Tiedong Wang, Yongxing AiFluorescence intensity of rhodamine released on the hydrolysis of the rhodamine-conjugated substrates FAT10 (A), ISG15 (B), NEDD8 (C), or Ufm1 (D). The fluorescence intensity traces of either chUSP1FL/chUAF1-containing reactions or negative controls containing an equal volume of buffer (Control) are shown for each substrate.
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chUSP 1 deubiquitination activitychUAF 1DUB ubiquitin-specific protease 191AUSP-associated factor 1chUSP 1NEDDsubstrate specificityMDVDNA damage repairsubstrate preferencesH 594A chUSP 1 mutantsSUMO 3-dimers ISG 15-Rho FAT 10-Rhodeubiquitination activity758AchUSP 1 mutants C 91S H 603ASUMO 1-GST SUMO 2-chicken USP 1 homologue
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