Hydrolysis of rhodamine-conjugated Ub-like substrates by chUSP1<sup>FL</sup> /chUAF1.

<p>Fluorescence intensity of rhodamine released on the hydrolysis of the rhodamine-conjugated substrates FAT10 (A), ISG15 (B), NEDD8 (C), or Ufm1 (D). The fluorescence intensity traces of either chUSP1<sup>FL</sup>/chUAF1-containing reactions or negative controls containing an equal volume of buffer (Control) are shown for each substrate.</p>