Fig 1.tif (561.1 kB)
Hydrolysis and transglycosylation reactions using SG-oxazoline as donor substrates by Endo-S mutants.
figure
posted on 2018-02-23, 18:54 authored by Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kensuke Nakamura, Yoshirou Kawaguchi, Takeshi Honda, Jun Hasegawa(A) Hydrolysis reactions were performed using trastuzumab and Endo-S mutants at a 50:1 weight ratio. (B) Transglycosylation reactions were carried out using deglycosylated trastuzumab as the acceptor and 5 molar eq of donor oxazoline.
