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Hydrogen Peroxide Displacing DNA from Nanoceria: Mechanism and Detection of Glucose in Serum
journal contribution
posted on 2016-01-11, 12:04 authored by Biwu Liu, Ziyi Sun, Po-Jung Jimmy Huang, Juewen LiuHydrogen
peroxide (H2O2) is a key molecule
in biology. As a byproduct of many enzymatic reactions, H2O2 is also a popular biosensor target. Recently, interfacing
H2O2 with inorganic nanoparticles has produced
a number of nanozymes showing peroxidase or catalase activities. CeO2 nanoparticle (nanoceria) is a classical nanozyme. Herein,
a fluorescently labeled DNA is used as a probe, and H2O2 can readily displace adsorbed DNA from nanoceria, resulting
in over 20-fold fluorescence enhancement. The displacement mechanism
instead of oxidative DNA cleavage is confirmed by denaturing gel electrophoresis
and surface group pKa measurement. This
system can sensitively detect H2O2 down to 130
nM (4.4 parts-per-billion). When coupled with glucose oxidase, glucose
is detected down to 8.9 μM in buffer. Detection in serum is
also achieved with results comparable with that from a commercial
glucose meter. With an understanding of the ligand role of H2O2, new applications in rational materials design, sensor
development, and drug delivery can be further exploited.