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Human prolactin physiology
thesis
posted on 2017-11-29, 04:20 authored by David Lindsay HealyThe overall aim of the work to be presented in this
thesis was to investigate the biology of the protein hormone prolactin
(PFL) in two areas of reproductive endocrinology:
hypothalamic-pituitary control of PRL secretion and the possible origin
of amniotic fluid PRL. For these studies, a reliable homologous PRL
double antibody radioimmunoassay was developed using materials supplied
by the NIAMDD. Serum PRL concentrations in 60 normal men were 12.8 ±
3.2 ng/ml (mean± 2 SO) and in 24 premenopausal women 13.6 ± 5.4 ng/ml.
Depending onthe iodination procedure used, the mean assay sensitivity
was 0.55 -0.61 ng/ml, the within-assay precision (expressed as a
coefficient of variation) was between 5.6 - 6.6 per cent and the
between-assay precision for medium pool samples was 16 - 19%. Factors
crucial to the development of a reliable assay were the use of minimal
oxidation conditions for PRL iodination, complete separation of the
iodinated species so formed, use of a highly dilute PRL antiserum to
increase assay sensitivity, careful titration of the amount of
non-immune serum used to increase ant genic bulk for the second antibody
step and a long incubation time to ensure maximal immunoprecipitation. [...]
History
Principal supervisor
Henry BurgerYear of Award
1979Department, School or Centre
Clinical Sciences at Monash HealthAdditional Institution or Organisation
Department of Obstetrics and Gynaecology and Prince Henry's Hospital, Medical Research CentreCampus location
AustraliaCourse
Doctor of PhilosophyDegree Type
DoctorateFaculty
Faculty of Medicine Nursing and Health SciencesUsage metrics
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