ac5b03522_si_001.pdf (1.5 MB)
Horseradish Peroxidase-Mediated, Iodide-Catalyzed Cascade Reaction for Plasmonic Immunoassays
journal contribution
posted on 2015-11-03, 00:00 authored by Yunlei Xianyu, Yiping Chen, Xingyu JiangThis
report outlines an enzymatic cascade reaction for signal transduction
and amplification for plasmonic immunoassays by using horseradish
peroxidase (HRP)-mediated aggregation of gold nanoparticles (AuNPs).
HRP-catalyzed oxidation of iodide and iodide-catalyzed oxidation of
cysteine is employed to modulate the plasmonic signals of AuNPs. It
agrees well with the current immunoassay platforms and allows naked-eye
readout with enhanced sensitivity, which holds great promise for applications
in resource-constrained settings.