pone.0204305.g005.tif (1.01 MB)
Histological and ELISA analysis of carotid arteries.
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posted on 2018-10-10, 20:10 authored by Robert Seifert, Michael T. Kuhlmann, Sarah Eligehausen, Friedemann Kiefer, Sven Hermann, Michael SchäfersSemi-quantitative intensity scores of the MMP-2, MMP-9, MRP14 and Mac-3 staining (n = 5) displayed as summed group score for DS, US and inside cuff regions (A). The summed group score is calculated by adding together the consensus reading results of all 5 animals, visualizing the results with respect to the performed staining and region (US, cuff or DS). Total MMP-2 (B) and MMP-9 (C) concentration normalised to protein level of right CCA US and DS region (n = 13) and left CCA as control (n = 14).
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CCAMMP -9 activitiesplaque phenotypesMMP -2 concentrationsMMP activity discriminatesDS plaquesshear stress-induced atherosclerosisMMP -2MMP inhibitor-based fluorescencevivo fluorescence reflectance imagingmurine atherosclerosis Purposeatherosclerotic plaque rupturesELISA1.06 plaque-to-background ratioMMP -9 concentrationsMMP inhibitor-based photoprobe Cy 5.5-AF
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