Histologic analysis of bone erosions in mice with collagen-induced arthritis (CIA) after treatment with IFN-β

<p><b>Copyright information:</b></p><p>Taken from "Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2004;6(3):R239-R249.</p><p>Published online 23 Mar 2004</p><p>PMCID:PMC416442.</p><p>Copyright © 2004 van Holten et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> Mice were treated with the indicated concentrations of IFN-β for 7 days starting when the first clinical signs of arthritis were observed. Hind paws were taken for histology. Bone erosions were scored in a blinded manner on a scale of 0 to 4 as described in Materials and methods. A significant reduction in bone erosions was observed in the animals treated with 2.5 μg and 1.25 μg IFN-β per injection/mouse in comparison with controls (= 0.02); results are means ± . Immunohistochemical analysis of the number of cells positive for RANKL (receptor activator of NF-κB ligand) and c-Fos after 7 days of IFN-β treatment in CIA mice. Sections were scored on a 4-point scale. RANKL staining; a decrease of RANKL-positive cells was observed in the group of animals treated with 2.5 μg IFN-β, although not statistically significant (= 0.07). c-Fos staining; the number of c-Fos-positive cells was significantly reduced in the mice treated with daily 2.5 μg per injection IFN-β (= 0.04). *Statistically significant difference. , standard error of the mean.