ac034395r_si_001.pdf (69.61 kB)
High-Throughput Analysis of Total RNA Expression Profiles by Capillary Gel Electrophoresis
journal contribution
posted on 2003-07-22, 00:00 authored by Wenwan Zhong, Edward S. YeungWe studied the expression profiles of total RNA in three
types of human tissues. Reverse transcription was performed on total RNA samples extracted from kidney,
normal breast, and breast tumor. Then, fluorophor-labeled cDNAs were synthesized by using random hexamers as primers for analysis by capillary gel electrophoresis. By overlaying the electropherograms and by
performing multivariate analysis, we found that the RNA
patterns were significantly different among the tissue
samples. The protocol utilizes only submicroliter volumes
of PCR products and is compatible with multiple capillary
DNA sequencing instruments. These CE-based RNA patterns provide an alternative to RNA microarrays for
expression profiling for understanding the molecular basis
of disease.