Hepatic Transcriptome Perturbations in Dairy Cows Fed Different Forage Resources

A subgroup of cows (n = 5/diet) was randomly selected for analysis of liver transcriptome profile. Liver biopsy was collected at the end of 14-week feeding to measure mRNA profiles using RNAseq. Analysis of differently expressed genes (DEG) was performed using DESeq2. Dynamic Impact Approach (DIA), Database for Annotation, Visualization and Integrated Discovery (DAVID), and ClueGO were used for bioinformatics analysis of DEG. There were 504 DEG with Padj ≤ 0.05 with 81 up-regulated and 423 down-DEG in CS vs. MF.