HeLa cells undergoing metaphase

2018-12-03T09:40:03Z (GMT) by Marie Nugent Laura O'Regan
Lay summary and brief biological or clinical significance
A human cancer cell is shown in the act of dividing. Understanding the process of cancer cell division and the molecular pathways involved allows us to identify new strategies for the selective killing of rapidly dividing cancer cells.

Technical summary
Human HeLa cervical carcinoma cells were fixed in ice-cold methanol and stained with antibodies against α-tubulin to detect microtubules (red) and CenpA to detect kinetochores (green). DNA was stained with Hoechst 33258 (blue) to identify the condensed chromosomes.

Imaging Details

Hardware

Leica Confocal TCS SP5

Image Capture Software

Leica Software

Laser(s) used
(if applicable)

50 mW diode 405 nm; 100 mW Argon laser 488; 10 mW DPSS 561 nm

Fluorophores used
(if applicable)

Primary: α-tubulin (Mouse); CenpA (Rabbit)

Secondary: Alexa fluor goat anti-rabbit 594; Alexa fluor goat anti-mouse 488, Hoechst 33258

Objective

X63 Plan Apo