HPIV3 IBs do not inhibit SG formation induced by AS or pIC.

(A-D) HeLa cells were transfected with an empty plasmid or co-transfected with plasmids encoding GFP-P and N-Myc for 24 h, then (A and B) treated with AS for another 1 h, or (C and D) transfected with pIC for another 12 h. (A and C) Cells were immunostained for G3BP (red). Nuclei were stained with DAPI (blue). The white scale bar corresponds to 10 μm. (B and D) The percentage of cells containing SGs was quantified in three independent experiments. Data are represented as means ±SD. Student’s t test: * P<0.05, ** P<0.01, *** P<0.001, ns = not significant.