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HIV+HCV co-culture increased mRNA and protein expression levels of α-SMA, collagen IV, TGF-β, and EPM in HSCs.

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posted on 2016-06-30, 18:08 authored by Lei Shi, Enqiang Qin, Junnian Zhou, Juanjuan Zhao, Weimin Nie, Tianjun Jiang, Weiwei Chen, Dan Wu, Lei Huang, Liying Liu, Liping Lv, Min Zhao, Zheng Zhang, Fusheng Wang

(A) QRT-PCR analysis of fibrogenesis-related genes in LX-2 cells. MRNA expression levels of α-SMA, collagen IV, TGF-β, and EPM were significantly upregulated after culture with HIV+HCV. GAPDH was used as the internal control. *P < 0.05 compared with the HIV or HCV group. (B) Western blotting analysis of the fibrogenesis-related protein expression levels in LX-2 cells. EPM, α-SMA, collagen IV, and TGF-β levels were upregulated after 72 h of co-culture with HIV+HCV. GAPDH was used as an internal control. (C) Western blotting analysis of EPM protein expression levels in LX-2 cells incubated with HIV+HCV at the indicated time points (h). GAPDH was used as an internal control. Bar graphs are shown on the right. * P < 0.05 compared with 24h.

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