Growth comparisons of 1291wt (■) and 1291<i>ackA</i> (●) from aerobically grown broth cultures.

<p>Samples were taken at 0, 1, 2, 3, 4, 5, 15, and 24 h, plated in triplicate, and the average colony forming units (CFU) determined for each time-point. Duplicate experiments were performed and the results presented are the average of those two values. As can be seen, aerobic growth of 1291<i>ackA</i> is limited in liquid media compared to its parent strain 1291wt.</p>