Fig 10.tif (2.76 MB)
Granulosa cells co-incubated with exosomes showed reduction in ROS level under oxidative stress or normal conditions compared to control ones.
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posted on 2017-11-08, 18:44 authored by Mohammed Saeed-Zidane, Lea Linden, Dessie Salilew-Wondim, Eva Held, Christiane Neuhoff, Ernst Tholen, Michael Hoelker, Karl Schellander, Dawit TesfayeThe ROS level (A) was measured under normal or oxidative stress conditions in granulosa cells co-incubated with exosomes released under normal (NormalExo) or stress (StressExo) conditions compared to groups cultured without exosomes. The ROS fluorescence intensity analysis (B). Data are mean ± SEM from four independent biological replicates. Bars with different letters showed statistically significant differences (p < 0.05).
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Bovine granulosa cellsoxidative stress responsecell cycle assaysgranulosa cellsoxidative stress response moleculesPRDXROSRNAcontrol group 24 hr post-treatmentcell cycle transitions5 μ M H 2 O 2exosomeprotein expression analysisDMEMexperiment sub-confluent cellsoxidative stress conditionsoxidative stressH 2 O 2TXNNrf 2
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