Gene gains and deletions in SM gene clusters.

(A) Differences in gene content in SM gene cluster 14 in Aspergillus fumigatus strains and closely related species. Four A. fumigatus strains lack an 11-kb region in this cluster, including an NRPS backbone gene, highlighted in yellow. Regions upstream and downstream of this cluster are syntenic. LMB35Aa also contains a large inversion that moves a transcription factor, oxidoreductase, and hypothetical protein 275 kb away from the cluster. A. fischeri and A. lentulus, close relatives of A. fumigatus, contain a cluster lacking the 11-kb region. (B) SM gene cluster found in most A. fumigatus strains but absent from the Af293 reference and from the F7763 strain. EOS, end of scaffold; MFS, major facilitator superfamily; NRPS, nonribosomal peptide synthase; SM, secondary metabolite.