GRA7-I and -III showed anti-mycobacterial activity against MTB challenge in vivo.

Schematic of the TB model treated with rGRA7 or vehicle (A, upper). (A) Bacterial loads in lung, liver, and spleen in PKCα+/+ and PKCα-/- mice. n = 10. (B) Histopathology scores were obtained from H&E stained lung sections (left), as described in Methods. Number of granulomas observed in 10 different lung sections per mouse (right). Scale bar, 500 μm. (C) Bacterial loads in lung, liver, and spleen in PLD1+/+ and PLD1-/- mice. n = 10. (D) Number of granulomas observed in 10 different lung sections per mouse. Black bars represent the median values. Student’s t-test and Grubbs’ outlier test were used for statistical analysis. The data are representative of two independent experiments with similar results. Significant differences (*P < 0.05; **P < 0.01; ***P < 0.001) compared with PKCα+/+ and PLD1+/+.