TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells treated with DMSO (control). Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. The data from these cells correspond to Supplementary Movie 3 and analysis of these movies provided data presented in Figure 3 and Supplementary Figure 4.
Supplementary Movie 3: A549 GFP-Rab11A cells under control treatment (DMSO).
Figure 3: Motion Dynamics of Rab11A under the effect of cytoskeletal depolymerizing drugs.
Supplementary Figure 4: Impact of skeletal depolymerizing drugs on the transport of Rab11A.
Funding
Visualizing Influenza Viral RNA Packaging
National Institute of Allergy and Infectious Diseases