np8b00787_si_002.pdf (2.2 MB)
Fusaristatin C, a Cyclic Lipodepsipeptide from Pithomyces sp. RKDO 1698
journal contribution
posted on 2018-12-07, 20:49 authored by Logan
W. MacIntyre, Douglas H. Marchbank, Hebelin Correa, Russell G. KerrA new cyclic lipodepsipeptide, fusaristatin
C (1),
was obtained from the fungus Pithomyces sp. RKDO
1698, which was isolated from the Caribbean octocoral Eunicea
fusca. The 2D structure of fusaristatin C was elucidated
using NMR spectroscopy and mass spectrometry, while the absolute configuration
of the sole chiral amino acid residue (l-serine) was determined
using Marfey’s method. 3-Hydroxy-2,11-dimethyltetradecanoic
acid (HDMT) was cleaved from 1, and the absolute configuration
at the C-3 position was determined using Mosher’s ester analysis.
Subsequent J-based configuration analysis of 1 allowed for assignment of the C-2 configuration. Fusaristatin
C exhibited no antimicrobial activity or cytotoxicity.