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Functionalization of Ultrabithorax Materials with Vascular Endothelial Growth Factor Enhances Angiogenic Activity
journal contribution
posted on 2016-10-07, 00:00 authored by David
W. Howell, Camille L. Duran, Shang-Pu Tsai, Sarah E. Bondos, Kayla J. BaylessSuccessful
design of tissue engineering scaffolds must include
the ability to stimulate vascular development by incorporating angiogenic
growth factors. Current approaches can allow diffusion of growth factors,
incorporate active factors randomly, or can leave residual toxins.
We addressed these problems by genetically fusing the gene encoding
Vascular Endothelial Growth Factor (VEGF) with the Ultrabithorax (Ubx)
gene to produce fusion proteins capable of self-assembly into materials.
We demonstrate that VEGF-Ubx materials enhance human endothelial cell
migration, prolong cell survival, and dose-dependently activate the
VEGF signaling pathway. VEGF-Ubx fibers attract outgrowing sprouts
in an aortic ring assay and induce vessel formation in a chicken embryo
chorioallantoic membrane (CAM) assay. Collectively, these results
demonstrate that the activity of VEGF remains intact in Ubx materials.
This approach could provide an inexpensive and facile mechanism to
stimulate and pattern angiogenesis.
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vessel formationgrowth factorsgene encoding Vascular Endothelial Growth FactorVEGF-Ubx materialsCAMfusion proteinsUbx materialscell migrationpattern angiogenesisUltrabithorax Materialschicken embryo chorioallantoic membraneoutgrowing sproutscell survivalVEGF-Ubx fiberstissue engineering scaffoldsaortic ring assayangiogenic growth factorsVascular Endothelial Growth Factor Enhances Angiogenic ActivityCurrent approaches
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