Fluorescently labeled claudin-5 (Cldn5) cross-over endocytosed via clathrin pathway.

Cocultures of monotransfected Madin-Darby canine kidney cells II expressing Cldn5 fluorescently labeled with mTurquoise2 (TRQ) or yellow fluorescent protein (YFP). Treatment with inhibitors of caveolae-formation (filipin III, FIII; 2 h), clathrin-mediated endocytosis (chlorpromazine, CPZ; 1 h) and dynamin (dynasore, Dyn; 2 h). Purple: endogenous Cldn1. Relative numbers of TRQ-Cldn5 vesicles after treatment with inhibitors; mean±SEM, n>50. *p<0.05, **p<0.01, ***p<0.001, compared to the untreated control; one-way ANOVA.