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Fluorescence correlation spectroscopy data for GFPcomp-labeled species (green) and mCherry-labeled species (red).

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posted on 2016-03-15, 00:21 authored by Sergiy Avilov, Julie Magnus, Stephen Cusack, Nadia Naffakh

The experimental autocorrelation data, fitted curves (in black), and residuals, are shown for an individual representative HEK-293T cell. A. Control cell transiently expressing MBD-GFPcomp and mCherry and infected with the WSN-wt virus; single-component translational diffusion model. Arrow points to the inflection point of the autocorrelation curve. B. Cell transfected with GFP1-10 and NP-mCherry and infected with the WSN-PB2-GFP11 virus. Two-component translational diffusion model.

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    nucleoprotein oligomersinfection cyclePB 2 subunitTime-Resolved VisualisationNearly-Native Influenzareplication cyclegenomic RNA segmentsvirus-encoded progeny RNPsprogeny vRNPstime lapse imagingvRNP componentsNP-mCherry fusion proteinsIndividual Componentshealth concernvRNP assemblyViral ribonucleoproteinsRNP assemblyVirus Progeny Ribonucleoproteinsinteractionoligomerization statusinfluenza WSN-PB 2-GFP virusLive Infected Cells Influenza virusespolymerasePB 2 protein

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