ao8b02765_si_001.pdf (375.54 kB)
Fluorescence-Based Ratiometric Nanosensor for Selective Imaging of Cancer Cells
journal contribution
posted on 2019-01-18, 09:15 authored by Kelsey
E. McNeel, Noureen Siraj, Nimisha Bhattarai, Isiah M. WarnerHerein, we report the synthesis and characterization
of a nanosensor developed from a group of uniform materials based
on organic salts (GUMBOS). This sensor is composed of three ionic
species, namely, fluorescein, rhodamine B, and tetradecyltrihexyl
phosphonium (P66614) ions. Nanoparticles prepared from
this three-component GUMBOS, termed nanoGUMBOS, exhibited increased
fluorescence at two wavelengths with increases in pH values. The ratio
of fluorescence emission corresponding to FL and RhB components of
these nanoGUMBOS also changed with pH, allowing ratiometric analysis
of pH through fluorescence measurements. Peak ratios were significantly
different at pH 5 and pH 7, indicating potential applications of this
nanomaterial as a cancer diagnostic tool. In this regard, in vitro
fluorescence microscopy was employed to image cancerous and normal
breast cells incubated with nanoGUMBOS. Fluorescence imaging of cancer
cells showed higher uptake of these nanoGUMBOS and more pervasive
fluorescence intensity in comparison with normal cells. Thus, the
synthesized novel nanomaterial shows a great potential for rapid visual
imaging of cancer cells.