Fig 1.TIF (553.94 kB)
Flow diagram of the main experimental design.
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posted on 2016-09-23, 17:38 authored by Mohamed Samy Yousef, Mohamed Ali Marey, Nina Hambruch, Hiroyuki Hayakawa, Takashi Shimizu, Hassan Ali Hussien, Abdel-Razek Khalifa Abdel-Razek, Christiane Pfarrer, Akio MiyamotoBOEC (bovine oviduct epithelial cells) monolayers co-cultured with sperm at different time points (3, 6, 12 and 24 h). PMNs (polymorphonuclear neutrophils) isolated from whole blood and co-cultured for 4 h with BOEC-conditioned media. The cells were collected to analyse immune-related gene expressions using real-time PCR.
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TNF expressionProstaglandin E 2sperm-BOEC bindingPGE 2 secretionoviduct epithelial cellsExogenous PGE 2PGE 2IL 10 Expressionssub-confluent BOEC monolayersPGE 2 productionPMN gene expressionprostaglandin E 21BOviduct Epithelial Cells Enhances TGFB 1anti-inflammatory environmentpro-inflammatory cytokine expressionIL 10TGFB 1increase anti-inflammatory cytokines expressionexogenous PGE 2Bovine Oviduct SpermBOEC media co-cultured
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