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Flexible and Efficient Eletrokinetic Stacking of DNA and Proteins at an HF Etched Porous Junction on a Fused Silica Capillary
journal contribution
posted on 2012-08-21, 00:00 authored by Zhi-Yong Wu, Fang Fang, Yan-Qin He, Ting-Ting Li, Jing-Jing Li, Li TianBetter understanding of the mechanism is important for
exploring
the potentials of a preconcentration method. In this work, we show
for the first time that the HF etched porous junction on a fused silica
capillary behaves not only as a filter but also as an integrated nanofluidic
interface. This junction exhibits an obvious ion concentration polarization
(CP) effect, with which highly efficient electrokinetic stacking (ES)
inside the capillary can be achieved without molecular size or charge
type limitation. Two major types of CP based ES were proposed, and
an autostop etching principle was presented for avoiding overetching.
The ES can be performed in a broad range of pH and buffer concentration.
Over a billion times of concentration was demonstrated by a fluorescein
probe with laser induced fluorescent (LIF) detection. ES of fluorescently
labeled and native DNA and protein were characterized by charge-coupled
device (CCD) imaging and online capillary gel electrophoresis (CGE)
with ultraviolet (UV) absorption detections, respectively. With this
junction, highly efficient ES can be performed easily by voltage manipulation
without any mechanical operation. We may foresee that the performance
of capillary-based conventional and chip electrophoresis could be
greatly enhanced with this junction in the analysis of low abundance
biomolecules.
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LIFabundance biomoleculesCCDvoltage manipulationfluorescein probecharge type limitationHF Etched Porous Junctionnanofluidic interfaceESUVCGEautostop etching principlecapillary gel electrophoresisDNACPsilica capillaryjunction exhibitsbuffer concentrationabsorption detectionsion concentration polarizationpreconcentration methodchip electrophoresisEfficient Eletrokinetic StackingFused Silica CapillaryBetter understanding
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