Figure S3: No effects on HIV-1 LTRtransactivation when parental U2OS cells were treated with doxycycline post infection from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

Hofmann, Stephan; Dehn, Sandra; Businger, Ramona; Bolduan, Sebastian; Schneider, Martha; Debyser, Zeger; Brack-Werner, Ruth; Schindler, Michael

doi:10.6084/m9.figshare.5436595.v1

rsob170115_si_003.pdf (19.31 kB)

Figure S3: No effects on HIV-1 LTRtransactivation when parental U2OS cells were treated with doxycycline post infection from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

journal contribution

posted on 2017-09-25, 10:46 authored by Stephan Hofmann, Sandra Dehn, Ramona Businger, Sebastian Bolduan, Martha Schneider, Zeger Debyser, Ruth Brack-Werner, Michael Schindler

U2OS cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRES-eGFP for 24 h. Thereafter, cells were treated with 1 μg/ml doxycycline.Additional 24 h later cells were analyzed by flow cytometry for the % and MFI of GFP+ (HIV-1-infected) cells. The graph shows mean values and standard deviations of three independent experiments.