SIM image reconstruction and registration log files and overlay image for mSin1.2-GFP (enhanced by Atto488-conjugated anti-GFP scAb) and mCherry-KRas4BC30.
The reconstructed SIM image file was split into 2 fluorescence channels and the contrast was adjusted across the entire image using ImageJ (Fiji built). No non-linear adjustments were made. Contrast settings: 50..7000 (PM); 1000..5000 (mSin1).