Figure S1

A) In preliminary test of skin exposure, lymphocyte subpopulations in the auricular lymph nodes and serum IgE level were measured from DMSO vehicle, 0.625%, 1.25%, 2.5%, and 5% cobalt treated Balb/c mice 7 days after dermal sensitization. n=4-7 per group. B) In preliminary test of respiratory challenge, differential cell count in bronchoalveolar lavage (BAL) were measured from four groups of saline, 0.0625%, 0.125%, 0.25%, and 0.5% cobalt treated Balb/c mice 24 h after one-time oropharyngeal challenge. n=3 per group. Data are presented as mean ± SD. *p<0.05 compared to the vehicle control group.