Figure S1; Figure S2; Figure S3 from Infection rate of <i>Aedes aegypti</i> mosquitoes with dengue virus depends on the interaction between temperature and mosquito genotype

Growth of DENV-2 strains in Aag-2 cells in tissue culture after 1, 3, 5, and 7 days post-infection, as measured by real-time PCR. Post hoc analysis (Tukey) revealed overall significant differences in virus titer between day 1 and the other time points ( zD1-D3=7.068, zD1-D5= 9.622, zD1-D7=8.141; p < 0.001) but no significant differences were detected among subsequent time points (zD3-D5=2.553, p=0.0521; zD3-D7=1.071, p=0.7071 and zD5-D7= -1.482, p=0.4485). Mean titers and 95% CI are represented by a red dot and corresponding whiskers.; Growth of DENV-2 strains in Aag-2 cells in tissue culture at 5 days post-infection as measured by real-time PCR. No significant differences in virus titer were detected between the DENV-Hanoi (filled boxes) and DENV-HCM strain (open boxes) across the three temperatures (F(d.f. 1,69)= 3.135; p < 0.0813). The box delimits the lower and upper quartiles of the data (25% and 75%). The darker horizontal line within the box represents the median and the whiskers are the minimum and maximum values.; Genotype by genotype by environment interactions between Aedes aegypti, DENV-2, and temperature. Alternative visual display to Figure 5. Dashed lines: Ho Chi Minh mosquitoes; Filled lines: Hanoi mosquitoes. Shaded areas are standard errors of the estimated sample proportion (SEp = sqrt [ p(1 - p) / n ]). Extrinsic incubation temperatures are represented by colors. Blue: 25oC; Green: 27oC, and Red: 32oC. Points are connected to facilitate visualization.