Figure S1. Expression pattern of Phospho anti-Histone H3 in the larval retina; Figure S2. Expression pattern of sense-control probes in the larval retina. from Eye development in the four-eyed fish Anableps anableps: cranial and retinal adaptations to simultaneous aerial and aquatic vision

A. anableps eye at Stage 3 (a,b), Stage 4 (c,d) and Stage 5 (e,f). Hematoxylin and eosin stained cryosections (a,c,e). Phospho anti-Histone H3 (S10) (green) and DAPI (blue) immunofluorescence staining (b,d,f) Panels on third column are high magnification images of the ventral and dorsal ciliary marginal zone (CMZ) of the retina. DR, dorsal retina; VR, ventral retina; L, lens; Ir, iris; DC, dorsal cornea; VC, ventral cornea; Do, dorsal; Ve, ventral; Di, distal; Pr, proximal. Cryosections 20 µm, scale bars 500 µm.; In situ hybridizations using sense probes of visual opsins in eye sections of larvae of A. anableps: rh1 (a,b), rh2-1 (c,d), rh2-2 (e,f), sws2b (g,h), and lws (i,j). Scale bars are 50 µm.