Figure 9: Effects of Acute VNS on NO Synthesis and Inflammation in the Lung

Values are mean ± SEM. Differences were tested by using Student’s t-test. ∗p < 0.05 and ∗∗p < 0.01 vs. SS. Effects of acute VNS (n = 3) or SS (n = 3) on nitric oxide (NO) synthesis in the lung of rats 5 weeks after SU5416 injection. Representative Western blotting of <b>(A)</b> endothelial nitric oxide synthase (eNOS) and <b>(B)</b> phosphorylated eNOS (p-eNOS) are shown in the upper panels. Graphs indicate semi-quantitative analysis of Western blots of eNOS and p-eNOS normalized by β-actin. In addition, effects of acute VNS (n = 4) or SS (n = 5) on messenger ribonucleic acid expressions of <b>(C)</b> <i>Il1b</i>, <b>(D)</b> <i>Il6</i>, <b>(E)</b> <i>Tnfa</i>, <b>(F)</b> <i>Mcp1</i>, <b>(G)</b> <i>Il10</i>, and <b>(H)</b> <i>Alpha7nachr</i> are shown; <i>18s</i> was used as the loading control. Each value was normalized by the averaged value in SS. Other abbreviations as in Figure 2.