Figure 9: Effects of Acute VNS on NO Synthesis and Inflammation in the Lung

Values are mean ± SEM. Differences were tested by using Student’s t-test. ∗p < 0.05 and ∗∗p < 0.01 vs. SS. Effects of acute VNS (n = 3) or SS (n = 3) on nitric oxide (NO) synthesis in the lung of rats 5 weeks after SU5416 injection. Representative Western blotting of (A) endothelial nitric oxide synthase (eNOS) and (B) phosphorylated eNOS (p-eNOS) are shown in the upper panels. Graphs indicate semi-quantitative analysis of Western blots of eNOS and p-eNOS normalized by β-actin. In addition, effects of acute VNS (n = 4) or SS (n = 5) on messenger ribonucleic acid expressions of (C) Il1b, (D) Il6, (E) Tnfa, (F) Mcp1, (G) Il10, and (H) Alpha7nachr are shown; 18s was used as the loading control. Each value was normalized by the averaged value in SS. Other abbreviations as in Figure 2.