Figure 2. Gene expression analysis of DPPC populations by RT-PCR, at early PDs.
2016-04-14T08:18:33Z (GMT)
by
<br>
<p>Gene
expression analysis of DPPC
populations by RT-PCR, at early PDs. All
DPPCs showed positive expression for the MSC markers, CD73, CD90 and CD105; and
the absence of the hematopoietic marker, CD45.
All DPPCs analysed were also negative for hTERT. Only low
proliferative capacity DPPCs (A1, A2, B1, C2 and C3) were positive for the
expression of CD271 (nerve growth factor receptor p75, LNGFR) and for all 3 senescence-associated
marker genes analysed, p53, p21<sup>waf1</sup>
and p16<sup>INK4a</sup>. Replacement of
cDNA with H<sub>2</sub>O served as negative controls, with β-actin serving as
the reference housekeeping gene.</p>
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